Electrophoretic techniques in the analysis of polydeoxycytidylic acids
| Authors | |
|---|---|
| Year of publication | 2012 |
| Type | Article in Proceedings |
| Conference | XII. Pracovní setkání fyzikálních chemiků a elektrochemiků. Sborník příspěvků |
| MU Faculty or unit | |
| Citation | |
| Field | Analytic chemistry |
| Keywords | electrophoresis oligonucleotides coated capillary |
| Description | Electrophoretic techniques such as capillary zone electrophoresis and one-dimensional polyacrylamide gel electrophoresis were applied in the separation of polydeoxycytidylic acids between 3 and 18 nucleotides in chain length (dC3-dC18). In capillary zone electrophoresis (CZE), the electrophoretic behavior of homo-oligonucleotides in hydrophilic coated capillary (CEP) and polyethyleneimine coated capillary (PEI) was followed under various experimental conditions. One-dimensional polyacrylamide gel electrophoresis was chosen as a supplemental method in the characterization of electrophoretic properties of polydeoxycytidylic acids. |
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