Piezoelectric and optical biosensors for detection of Bacillus atrophaeus
| Authors | |
|---|---|
| Year of publication | 2012 |
| Type | Article in Proceedings |
| Conference | XII. Pracovní setkání fyzikálních chemiků a elektrochemiků |
| MU Faculty or unit | |
| Citation | |
| Web | http://web2.mendelu.cz/af_239_nanotech/data/up/sbornik-mendelu.pdf#page=43 |
| Field | Biochemistry |
| Keywords | Bacillus atrophaeus; Biosensor; QCM; SPR |
| Description | Interactions of antibodies with the bacterium Bacillus atrophaeus were studied using piezoelectric (PZ) and surface plasmon resonance (SPR) based biosensors, with the aim of future development of biosensor for Bacillus species. Antibodies were covalently immobilised on gold surfaces of PZ crystal resonators and SPR chips. QCM Analyzer and Biacore 3000 were used for real-time and label-free measurements of immunocomplexes. Concentrations of B. atrophaeus below 10^5 CFU ml-1 were successfully detected. |
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