Arrangement of nuclear structures is not transmitted through mitosis but is identical in sister cells

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Authors

ORLOVA Darya Yurevna STIXOVÁ Lenka KOZUBEK Stanislav GIERMAN Hinco ŠUSTÁČKOVÁ Gabriela CHERNYSHEV Andrei MEDVEDEV Ruslan LEGARTOVÁ Soňa VERSTEEG Rogier MATULA Pavel STOKLASA Roman BÁRTOVÁ Eva

Year of publication 2012
Type Article in Periodical
Magazine / Source Journal of Cellular Biochemistry
MU Faculty or unit

Faculty of Informatics

Citation
Web http://onlinelibrary.wiley.com/doi/10.1002/jcb.24208/abstract
Doi http://dx.doi.org/10.1002/jcb.24208
Field Genetics and molecular biology
Keywords histones; chromatin; HP1 protein; photoconversion; Dendra2; Cajal bodies
Description Although it is well known that chromosomes are non-randomly organized during interphase, it is not completely clear whether higher-order chromatin structure is transmitted from mother to daughter cells. Therefore, we addressed the question of how chromatin is rearranged during interphase and whether heterochromatin pattern is transmitted after mitosis. We additionally tested the similarity of chromatin arrangement in sister interphase nuclei. We noticed a very active cell rotation during interphase, especially when histone hyperacetylation was induced or transcription was inhibited. This natural phenomenon can influence the analysis of nuclear arrangement. Using photoconversion of Dendra2-tagged core histone H4 we showed that the distribution of chromatin in daughter interphase nuclei differed from that in mother cells. Similarly, the nuclear distribution of heterochromatin protein 1beta (HP1beta) was not completely identical in mother and daughter cells. However, identity between mother and daughter cells was in many cases evidenced by nucleolar composition. Moreover, morphology of nucleoli, HP1beta protein, Cajal bodies, chromosome territories, and gene transcripts were identical in sister cell nuclei. We conclude that the arrangement of interphase chromatin is not transmitted through mitosis, but the chromatin pattern is identical in naturally synchronized sister cells. It is also necessary to take into account the possibility that cell rotation and the degree of chromatin condensation during functionally specific cell cycle phases might influence our view of nuclear architecture.
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