High resolution positioning of intron ends on the nucleosomes

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Authors	HAPALA Jan TRIFONOV Eduard
Year of publication	2011
Type	Article in Periodical
Magazine / Source	Gene
MU Faculty or unit	Central European Institute of Technology
Citation
Doi	http://dx.doi.org/10.1016/j.gene.2011.08.022
Field	Genetics and molecular biology
Keywords	AG-ends of introns; Gene splicing; GT-ends of introns; Nucleosome mapping; Protection of splice junctions; Rotational positioning
Description	High resolution sequence-directed nucleosome mapping is applied to 36,000 sequences containing splice junctions, from five different species.Moreover, the orientation of guanine residues at the GT- and AG-ends of introns within the nucleosomes is such that the guanines are positioned nearest to the surface of histone octamers, 3 and 4 bases upstream from the local DNA pseudo-dyads passing through minor grooves oriented outwards. Since the guanine residues are the most vulnerable to spontaneous damage within the cell (primarily, depurination and oxidation) such positioning of the splice junctions minimizes the damage that is caused by free radicals and highly reactive metabolites.
Related projects:	Molecular basis of cell and tissue regulations CHROMPLANT