Microfluidic robotic device coupled with electrochemical sensor field for handling of paramagnetic micro-particles as a tool for determination of plant mRNA.

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Authors

HUSKA Dalibor ADAM Vojtěch BABULA Petr TRNKOVÁ Libuše HUBÁLEK Jaromír ZEHNÁLEK Josef HAVEL Ladislav KIZEK René

Year of publication 2011
Type Article in Periodical
Magazine / Source Microchim. Acta
MU Faculty or unit

Faculty of Science

Citation
Web https://link.springer.com/article/10.1007/s00604-011-0545-z
Doi http://dx.doi.org/10.1007/s00604-011-0545-z
Field Physical chemistry and theoretical chemistry
Keywords Electrochemical detection; Microfluidics; Environmental stress; Cadmium ions; Paramagnetic particles
Description The expression of genes responsible for the biosynthesis of stress proteins corresponds to the exposition of an organism to abiotic and/or biotic stress. We utilize two types of paramagnetic particles for isolation of total mRNA from early somatic embryos of Norway Spruce (Picea abies /L./ Karst.) and maize plants (Zea mays L.) treated with cadmium(II) ions. The paramagnetic particles were evaluated for analysis of real samples, and polyadenine was used as a model mRNA. Various approaches (from non-automatic to fully automatic) were tested in terms of handling the particles.
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