Sample preparation methods for MALDI-MS profiling of bacteria

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Authors

ŠEDO Ondrej SEDLÁČEK Ivo ZDRÁHAL Zbyněk

Year of publication 2011
Type Article in Periodical
Magazine / Source Mass Spectrometry Reviews
MU Faculty or unit

Central European Institute of Technology

Citation
Doi http://dx.doi.org/10.1002/mas.20287
Field Analytic chemistry
Keywords MALDI-MS; bacteria profiling; microorganism identification
Description Direct matrix-assisted laser desorption/ionization mass spec- trometry (MALDI-MS) bacterial cell or lysate analysis appears to meet all the criteria required for a rapid and reliable analytical microorganism identification and taxonomical classification tool. Few-minute analytical procedure providing information extending up to sub-species level underlines the potential of the MALDI-MS profiling in comparison with other methods employed in the field. However, the quality of MALDI-MS profiles and consequently the performance of the method are influenced by numerous factors, which involve particular steps of the sample preparation procedure. This review is aimed at advances in development and optimization of the MALDI-MS profiling methodology. Approaches improving the quality of the MALDI-MS profiles and universal feasibility of the method are discussed.
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