WNT unrelated activities in commercially available preparations of recombinant WNT3a
| Title in English | WNT unrelated activities in commercially available preparations of recombinant WNT3 |
|---|---|
| Authors | |
| Year of publication | 2010 |
| Type | Article in Periodical |
| Magazine / Source | Journal of Cellular Biochemistry |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | WNT pathway; ligand; purification; signaling; AKT/GSK3; phosphorylation; SFRP1; beta-catenin |
| Description | WNT signaling pathways play an important role in both development and disease. By analyzing the signaling capabilities of commercially available WNT3a preparations towards the PI3K/AKT/GSK3 signaling pathway, we discovered unexpected inconsistencies from lot to lot of recombinant WNT3a. We provide evidence that: (1) The ability to trigger AKT/GSK3 signaling varies dramatically between different lots of WNT3a, without any variation in their ability to activate the canonical WNT/beta-catenin signaling. (2) sFRP1, a WNT signaling inhibitor, is unable to interfere with the activation of AKT/GSK3 signaling induced by some of the WNT3a lots. (3) Pharmacological inhibition of AKT/GSK3 phosphorylation by PI3K inhibitors fails to affect the stabilization of beta-catenin, the central effector of the canonical WNT/beta-catenin signaling pathway. |
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