Gene expression profiling in follicular lymphoma and its implication for clinical practise

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Authors	JANÍKOVÁ Andrea TICHÝ Boris ŠUPÍKOVÁ Jana STAŇO KOZUBÍK Kateřina POSPÍŠILOVÁ Šárka KŘEN Leoš VÁŠOVÁ Ingrid ŠÁLEK David MAYER Jiří
Year of publication	2011
Type	Article in Periodical
Magazine / Source	LEUKEMIA & LYMPHOMA
MU Faculty or unit	Central European Institute of Technology
Citation
Doi	http://dx.doi.org/10.3109/10428194.2010.531412
Field	Oncology and hematology
Keywords	follicular lymphoma; gene expression profiling; lymphomagenesis; prognosis
Description	Follicular lymphoma(FL) is characterized by an indolent and relapsing course. Recently, the clinical outcome of FL has been distinguished by immune microenvironment associated gene signatures. In our study, gene expression profiling (GEP) was performed in 31 nonselected patients with follicular lymphoma (FL), 12 of whom were in relapse and the remaining 19 newly diagnosed. A custom oligonucleotide microarray (Agilent 8 x 15K) was used which contained probes for about 3500 genes, including those that had been previously published as demonstrating significant prognostic value. An unsupervised approach was not able to recognize clinically different FLs. As the previously published prognostically relevant gene signatures could not be properly verified, probably due to microarray platform differences, template matching was therefore used in order to define two gene sets with differential gene expression among our samples. These gene sets shared an overrepresentation of genes with similar biological functions and were termed T CELL and PROLIFERATION profiles. The poor profile was then defined by a high PROLIFERATION score (upper tertile) and or low T CELL score (lower tertile). The poor profile cohort contained a significantly higher proportion of relapsed cases (p 0,05 Fishers exact test). Additionally, a comparison of samples from initial diagnosis and from relapse showed significant differences mainly in the T CELL profile (p=0,036 x (2)). This supports the hypothesis that the number of T cells and their expression pattern play a major role in FL development.
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