125I-labelled iodothyronines: useful tools for studies of effects of an antidepressant drug fluoxetine in the rat
| Authors | |
|---|---|
| Year of publication | 2010 |
| Type | Article in Periodical |
| Magazine / Source | Journal of Radioanalytical and Nuclear Chemistry |
| MU Faculty or unit | |
| Citation | |
| Field | Pharmacology and pharmaceutical chemistry |
| Keywords | Antidepressant drug; radiometric enzyme assay; thyroid hormone |
| Description | We followed the effects of an antidepressant drug fluoxetine (Fluox), administered sub-chronically to Wistar rats by itself, or in combination with 3,3',5-triiodothyronine. In studies of the interaction of Fluox with the metabolism of thyroid hormones, we applied adapted radiometric enzyme assays for iodothyronine sulfotransferases and uridine 5'-diphospho-glucuronyltransferase, as well as our newly developed radiometric assays for iodothyronine deiodinases of types 1, 2 and 3, using 125I-labelled iodothyronines of high specific radioactivity as substrates. |
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