HPLC determination of caffeine and paraxanthine in saliva as a method for CYP1A2 metabolic activity assessment in humans
| Authors | |
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| Year of publication | 2010 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | The mehod for simple and reliable determination of caffein and paraxanthine in human saliva was developed. After simple, liquid-liquid extraction into organic solvents, sample were separated on Kinetex PFP column and detected at 268 nm. Mobile phase (0.05% acetic acid: methanol, 75:25) was eluted isocratically at flow rate of 0.55 ml/min. All analytes wre eluted within 13 min. |
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