Optimization of immunomagnetic selection of myeloma cells from bone marrow using magnetic activated cell sorting

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Authors

ČUMOVÁ Jana KOVÁŘOVÁ Lucie POTÁČOVÁ Anna BUREŠOVÁ Ivana KRYUKOV Fedor PENKA Miroslav MICHÁLEK Jaroslav HÁJEK Roman

Year of publication 2010
Type Article in Periodical
Magazine / Source International journal of hematology
MU Faculty or unit

Faculty of Medicine

Citation
Web http://www.ncbi.nlm.nih.gov/pubmed/20694533
Field Oncology and hematology
Keywords multiple myeloma; immunomagnetic separation; plasma cell; CD138
Description Plasma cells (PCs) enrichment from bone marrow samples of multiple myeloma (MM) patients is frequently performed by immunomagnetic separation (magnetic activated cell sorting, MACS) using anti-CD138 MicroBeads. The aim of our work was to find optimal strategy for immunomagnetic separation of PCs and determine optimal algorithm of separation techniques for samples with various percentage of neoplastic cells.
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