Proteomics uncovers extreme heterogeneity in the Staphylococcus aureus exoproteome due to genomic plasticity and variant gene regulation

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Authors	ZIEBANDT Anne-Kathrin KUSCH Harald DEGNER Marco JAGLITZ Sarah SIBBALD Mark J. J. B. ARENDS Jan P. CHLEBOWICZ Monika A. ALBRECHT Dirk PANTŮČEK Roman DOŠKAŘ Jiří ZIEBUHR Wilma BRÖKER Barbara M. HECKER Michael VAN DIJL Jan Maarten ENGELMANN Susanne
Year of publication	2010
Type	Article in Periodical
Magazine / Source	Proteomics
MU Faculty or unit	Faculty of Science
Citation
Web	http://www3.interscience.wiley.com/journal/123299148/abstract
Field	Genetics and molecular biology
Keywords	Exoproteome; Microbiology; Protein secretion; Staphylococcus; TOXIC-SHOCK-SYNDROME; METHICILLIN-RESISTANT; BACILLUS-SUBTILIS; SIGNAL-TRANSDUCTION; VIRULENCE FACTORS; NASAL CARRIAGE
Description	Sequencing of at least 13 Staphylococcus aureus isolates has shown that genomic plasticity impacts significantly on the repertoire of virulence factors. However, genome sequencing does not reveal which genes are expressed by individual isolates. Here, we have therefore performed a comprehensive survey of the composition and variability of the S. aureus exoproteome. This involved multilocus sequence typing, virulence gene, and prophage profiling by multiplex PCR, and proteomic analyses of secreted proteins using 2-DE. Dissection of the exoproteomes of 25 clinical isolates revealed that only seven out of 63 identified secreted proteins were produced by all isolates, indicating a remarkably high exoproteome heterogeneity within one bacterial species. Most interesting, the observed variations were caused not only by genome plasticity, but also by an unprecedented variation in secretory protein production due to differences in transcriptional and post-transcriptional regulation. Our data imply that genomic studies on virulence gene conservation patterns need to be complemented by analyses of the extracellular protein pattern to assess the full virulence potential of bacterial pathogens like S. aureus. Importantly, the extensive variability of secreted virulence factors in S. aureus also suggests that development of protective vaccines against this pathogen requires a carefully selected combination of invariably produced antigens.
Related projects:	Molecular basis of cell and tissue regulations