Analysis of DNA-binding proteins by FRAP AND FLIP

Dvořáčková,  Martina; Rossignol,  Pascale; Doonan, John; Shaw, Peter; Fajkus,  Jiří

Analysis of DNA-binding proteins by FRAP AND FLIP

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Authors	DVOŘÁČKOVÁ Martina ROSSIGNOL Pascale DOONAN John SHAW Peter FAJKUS Jiří
Year of publication	2009
Type	Article in Proceedings
Conference	Bulletin České společnosti experimentální biologie rostlin a Fyziologické sekce Slovenské botanické společnosti
MU Faculty or unit	Faculty of Science
Citation
Field	Genetics and molecular biology
Keywords	FRAP; dynamics; AtTRB1; telomeres
Description	High advanced microscopy represents an inevitable tool for analysis of molecular interactions. DNA binding properties of putative telomere binding AtTRB1 protein from Arabidopsis were analysed by FRAP and FLIP. We showed that AtTRB1 is highly dynamic chromatin components with fast mobility within the cell.
Related projects:	Molecular basis of cell and tissue regulations