Protein engineering of carbohydrate binding proteins - understanding the recognition phenomena

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Authors

WIMMEROVÁ Michaela

Year of publication 2009
Type Article in Proceedings
Conference FEBS Journal
MU Faculty or unit

Faculty of Science

Citation
Field Biophysics
Keywords protein engineering;lectin; pathogen
Description The contribution is focused on few examples where a combination of experimental methods (isothermal titration microcalorimetry, surface plasmon resonance, X-ray crystallography) with computational approaches can bring a significant profit for better understanding of protein-carbohydrate interactions on molecular level. Insight into structure correlation with function analysis of high-affinity lectins allows for construction of carbohydrate-binding proteins with tuned affinity and specificity for biotechnology and bioanalytical purposes.
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