) Subcellular homeostasis of phytohormone auxin is mediated by the ER-localized PIN5 transporter

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Authors

MRAVEC Jozef SKŮPA Petr BAILLY Aurélien HOYEROVÁ Klára KŘEČEK Pavel BIELACH Agnieszka PETRÁŠEK Jan ZHANG Jing GAYKOVA Vassilena STIERHOF York-Dieter DOBREV Petre I. SCHWARZEROVÁ Kateřina ROLČÍK Jakub SEIFERTOVÁ Daniela LUSCHNIG Christian BENKOVÁ Eva ZAŽÍMALOVÁ Eva GEISLER Markus FRIML Jiří

Year of publication 2009
Type Article in Periodical
Magazine / Source Nature
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords auxin; PIN5; polar auxin transport; endoplasmic reticulum; subcellular homeostasis
Description The plant signalling molecule auxin provides positional information in a variety of developmental processes by means of its differential distribution (gradients) within plant tissues. Thus, cellular auxin levels often determine the developmental output of auxin signalling. Conceptually, transmembrane transport and metabolic processes regulate the steady-state levels of auxin in any given cell. In particular, PIN auxin-efflux-carrier-mediated, directional transport between cells is crucial for generating auxin gradients. Here we show that Arabidopsis thaliana PIN5, an atypical member of the PIN gene family, encodes a functional auxin transporter that is required for auxin-mediated development. PIN5 does not have a direct role in cell-to-cell transport but regulates intracellular auxin homeostasis and metabolism. PIN5 localizes, unlike other characterized plasma membrane PIN proteins, to endoplasmic reticulum (ER), presumably mediating auxin flow from the cytosol to the lumen of the ER. The ER localization of other PIN5-like transporters (including the moss PIN) indicates that the diversification of PIN protein functions in mediating auxin homeostasis at the ER, and cell-to-cell auxin transport at the plasma membrane, represent an ancient event during the evolution of land plants.
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