c-Myb prevents apoptosis of colon carcinoma cells in Cox-2-independent manner

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Authors

KNOPFOVÁ Lucia NEŠPOROVÁ Kristina BENEŠ Petr ŠMARDA Jan

Year of publication 2009
Type Article in Proceedings
Conference International Journal of Molecular Medicine
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords apoptosis colon carcinoma c-Myb cyclooxygenase-2 cisplatin TRAIL
Description Development of the colon tumors is associated with mutations in various protooncogenes and tumor suppressor genes. c-myb codes for transcription factor that is involved in colorectal cancerogenesis. c-myb expression is frequently up-regulated in colon carcinomas compared to non-malignant colonic mucosa. The set of the Myb-targeted genes includes the cox-2 gene coding for cyclooxygenase 2. The aim of this study is to clarify the role of c-Myb and Cox-2 cooperation in regulation of apoptosis of colon carcinoma cell lines HCT116 and HT-29. We prepared HCT116 cells overexpressing c-Myb and HT-29 cells with silenced c-myb expression and confirmed that up- and down-regulation of c-Myb correlate with expression of cox-2. Next, we studied the role of c-Myb/Cox-2 in regulation of apoptosis in response to cis-platin and TRAIL. We showed that overexpression of c-myb stimulates resistance, while silencing of c-myb stimulates sensitivity of the colon carcinoma cells to both cis-platin and TRAIL.
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