HPLC Measurement of Thiamine and its Phosphorylated Forms in Whole Blood and Plasma: Thiamine Status Assessment

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Authors

TOMANDLOVÁ Marie TOMANDL Josef KAŇKOVÁ Kateřina

Year of publication 2009
Type Conference abstract
MU Faculty or unit

Faculty of Medicine

Citation
Description A new gradient ion-paired HPLC utilising fluorescence detection for the simultaneous determination of thiamine and its mono- and diphosphate esters in human whole blood and plasma is developed. The method has been sufficient for the accurate, reliable and reproducible determination of thiamine status in healthy persons an also patients with diabetic nephropathy.
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