DNA MIKROČIPY (MICROARRAYS) V DIAGNOSTICE FOLIKULÁRNÍHO LYMFOMU: CESTA K TERAPII „ŠITÉ NA MÍRU“ NEBO SLEPÁ ULIČKA?
| Title in English | DNA microchips (microarrays) in diagnostics of follicular lymphoma: the journey to tailored therapy or to impasse? |
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| Authors | |
| Year of publication | 2008 |
| Type | Article in Periodical |
| Magazine / Source | Transfuze a hematologie dnes |
| MU Faculty or unit | |
| Citation | |
| Keywords | follicular lymphoma, diagnosis, prognosis, gene expression profiling, DNA microarrays |
| Description | Follicular lymphoma (FL), an indolent B-lymphoproliferative disorder, is characterized by a long median survival of 8 to 10 years. Transformation to more aggressive lymphoma with poor prognosis occurs in 25-60% of patients with FL. Up to now; FL is considered to be an incurable disease with unpredictable individual course. Translocation t(14;18), the traditional genetic hallmark of FL, is of limited clinical importance only. Prognostic and laboratory parameters actually used have not been successful in individual stratification of FL patients as well. DNA microarrays enables analyses of the genomic expression program providing new insights into molecular pathogenesis of tumours. Gene expression profiling can reveal morphologically indistinguishable subgroups with different clinical behaviour in the frame of single disease, new important genes or prognosis based on molecular features. In principle, DNA microarrays are based on hybridization of tested nucleic acids (in expression microarrays RNA) with complementary oligonucleotide probes immobilized on the matrix of microarrays. The quantity of data generated by these techniques (usually thousands to tens of thousands genes) requires statistical analysis. There are several studies analysing gene expression profiling in FL from various aspects, published results generally indicate, that expression profiling of FL is relatively homogenous, transformed FL is clonally identical with its original indolent counterpart and its expression signature is more similar to those observed in FL than to those observed in de novo DLBCL. Transformation is heterogeneous process proceeded probably by at least two pathways. The nature of infiltrating non-B-cells of microenvironment seems to be of a great importance, concerning behaviour and prognosis of FL. |
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