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Title in English	Isolation of Plasma Cells
Authors	ČUMOVÁ Jana BUREŠOVÁ Ivana KOVÁŘOVÁ Lucie KYJOVSKÁ Drahomíra VIDLÁKOVÁ Petra SUSKÁ Renata PERUTKA Tomáš MORAVCOVÁ Jana RYCOVÁ Marcela PENKA Miroslav HÁJEK Roman
Year of publication	2008
Type	Article in Periodical
Magazine / Source	Klinická onkologie Supplement 2008
MU Faculty or unit	Faculty of Medicine
Citation
Field	Oncology and hematology
Keywords	Multiple myeloma;Separation;MACS;FACS;CD138
Description	We have used magnetic-activated cell sorting (MACS) and/or fluorescence-activated cell sorting (FACS) for the separation of plasma cells (PC) from bone marrow aspirates from patients with multiple myeloma (MM) using human antibodies CD138 MACS Micro- Beads or CD138-PE antibodies that recognize the plasma cell surface antigen CD138 (syndecan-1). Isolated PC can be used for molecular biology studies of MM such as PCR analysis, comparative genomic hybridization (CGH), fluorescence in situ hybridization (FISH), protein analysis, analysis of cell surface markers, and/or intracellular antigens.
Related projects:	University Research Centre - Czech Myeloma Group From classic prognostic markers to clinical applications in selected pharmacogenomic and pharmacoproteomic projects in multiple myeloma and monoclonal gammapathies