Evaluation of (GTG)5-PCR for rapid identification of Streptococcus mutans
| Authors | |
|---|---|
| Year of publication | 2008 |
| Type | Article in Periodical |
| Magazine / Source | Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology |
| MU Faculty or unit | |
| Citation | |
| Web | http://www.springerlink.com/content/268q817531426227/?p=e909337e76ae416fbddd48f749191788&pi=7 |
| Field | Microbiology, virology |
| Keywords | Dental caries; (GTG)5-PCR; Identification; Automated ribotyping; Streptococcus mutans |
| Description | Repetitive sequence-based polymerase chain reaction (PCR) fingerprinting using the (GTG)5 primer was applied for fast screening of bacterial strains isolated from dental plaque of early childhood caries (ECC)-affected children. A group of 29 Gram-positive bacteria was separated into a homogeneous cluster together with Streptococcus mutans reference strains and constituted an aberrant branch after the numerical analysis of (GTG)5-PCR fingerprints. Automated ribotyping with EcoRI restriction enzyme (RiboPrinter microbial characterization system) revealed high genetic heterogeneity among the tested group and proved to be a good tool for strain-typing purposes. Further characterization of the studied strains was achieved by extensive phenotyping and whole-cell protein fingerprinting and confirmed all the strains as S. mutans representatives. Obtained results showed rep-PCR fingerprinting with the (GTG)5 primer to be a fast and reliable method for identification of S. mutans. |
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