Optimization of polymerase chain reaction for detection of live and dead probiotic lactobacillus cells
| Authors | |
|---|---|
| Year of publication | 2008 |
| Type | Article in Proceedings |
| Conference | International Probiotic Conference. Pediatria 2, 2008. |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | ethidium monoazide; dead and live bacterial cells |
| Description | The general application of ethidium monoazide (EMA) is based on penetration into dead cells with copromised membrane integrity. DNA bound to EMA cannot be PCR amplified. Experiments were performed using probiotic bacterial strein Lactobacillus paracasei subsp. paracasei CCDM 211/06. The lactobacillus species specific primers were used for DNA amplification. The PCR products (250 bp) were separated using electrophoresis in 1.8 % agarose gel. The method was applied for live and dead lactobacillus cells identification in dairy products. |
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