Využití PCR při rozlišení živých a mrtvých bakteriálních buněk

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Title in English Differentiation of live and dead bacterial cells by PCR
Authors

TRACHTOVÁ Štěpánka ŠPANOVÁ Alena RITTICH Bohuslav

Year of publication 2008
Type Article in Proceedings
Conference XII. Setkání biochemiků a molekulárních biologů
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords PCR; EMA; live and dead cells;
Description Lactobacillus paracasei ssp.paracasei CCDM211/06 were used for confirmation of the method. Different methods were used for preparation of dead cells (control). The time of incubation of cell suspension with EMA as the time of irradiation of the sample with halogen lamp were tested. PCR was carried out using genus specific primers (Dubernet a spol., 2002).
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