High-Resolution Image Cytometry of a Large Number of 3D Confocal FISH and Immunofluorescence Stained Cells

Matula,  Pavel; Maška,  Martin; Daněk,  Ondřej; Matula,  Petr; Stejskal,  Stanislav; Ondřej, Vladan; Kozubek,  Michal

High-Resolution Image Cytometry of a Large Number of 3D Confocal FISH and Immunofluorescence Stained Cells

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Authors	MATULA Pavel MAŠKA Martin DANĚK Ondřej MATULA Petr STEJSKAL Stanislav ONDŘEJ Vladan KOZUBEK Michal
Year of publication	2008
Type	Conference abstract
MU Faculty or unit	Faculty of Informatics
Citation
Description	We have developed a system for high-resolution image cytometry of a large number of 3D confocal images of cells stained by fluorescent dyes. The system is a successor of a previously developed system for high-resolution image cytometry of FISH-stained cells. The new system, called Acquiarium, is capable of fast 3D acquisition of confocal images using microscopes with spinning disks and provides means for convenient analysis of many 3D image data in one collection. Features and the applicability of the new system were discussed.
Related projects:	Determination of markers, screening and early diagnostics of cancer diseases using highly automated processing of multidimensional biomedical images