Pyrosequencing as a tool for whole genome sequencing of Treponema pallidum subspecies.

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Authors

ČEJKOVÁ Darina MATĚJKOVÁ Petra STROUHAL Michal ZOBANÍKOVÁ Marie ŠMAJS David WEINSTOCK George M.

Year of publication 2007
Type Article in Periodical
Magazine / Source Chemické Listy
MU Faculty or unit

Faculty of Medicine

Citation
Web http://www.chemicke-listy.cz/docs/full/2007_05_425-465.pdf
Field Genetics and molecular biology
Keywords pyrosequencing; Treponema pallidum
Description Treponema pallidum includes several obligate human pathogens. T. p. pallidum is an agent of sexually transmitted syphilis, T. p. pertenue and T. p. endemicum causes yaws and endemic syphilis, respectively. Pyrosequencing (454 sequencing) is a massivelyparallel sequencing-by-synthesis system. In this study, genomic DNA of T. p. pertenue CDC-2 was completely sequenced using 454 technology.
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