3.	Structural and thermodynamical characterization of BclA, a lectin from Burkholderia cepacia complex (BCC)

Lameignere, Emilie; Malinovská,  Lenka; Varrot, Annabelle; Sláviková,  Margita; Mitchell, Edward P.; Wimmerová,  Michaela; Imberty, Anne

3. Structural and thermodynamical characterization of BclA, a lectin from Burkholderia cepacia complex (BCC)

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Title in English	Structural and thermodynamical characterization of BclA, a lectin from Burkholderia cepacia complex (BCC)
Authors	LAMEIGNERE Emilie MALINOVSKÁ Lenka VARROT Annabelle SLÁVIKOVÁ Margita MITCHELL Edward P. WIMMEROVÁ Michaela IMBERTY Anne
Year of publication	2007
Type	Article in Proceedings
Conference	14th European Carbohydrate Symposium, EUROCARB 14
MU Faculty or unit	Faculty of Science
Citation
Field	Biochemistry
Keywords	structure; lectin; pathogen; cepacia complex
Description	Printed Glycan arrays performed by the Consortium for Functional Glycomics (CFG) demonstrated that BclA has a strict specificity for oligosaccharide with a terminal alpha-mannose residue. Thermodynamics of the interaction of BclA with methyl-mannoside and some other mannosylated oligosaccharides was studied by isothermal titration microcalorimetry (ITC). protein has been crystallized and the structure of the complex with the methyl-mannoside was solved at 1.7A resolution.
Related projects:	Proteins in metabolism and interaction of organisms with the environment Biomolecular centre