Multidetection Platform for Microcolumn Separation of Proteins and Peptides
| Authors | |
|---|---|
| Year of publication | 2007 |
| Type | Article in Proceedings |
| Conference | Book of Abstracts and Abbreviated Papers 13th ISSS & 13th AACI |
| MU Faculty or unit | |
| Citation | |
| Field | Analytic chemistry |
| Keywords | microcolumn; separation; mass spectrometry; MALDI; CE; HPLC; fluorescence; LIF; inductively-coupled plasma |
| Description | The lecture describes an instrumentation platform based on a universal interface for deposition of CE or microLC eluent on a target of a MALDI mass spectrometer. The deposited fractions of proteins or peptides can be analyzed in off-line mode using MALDI mass or tandem mass spectrometry (MS or MS/MS) and native laser-induced fluorescence (LIF) detection or subjected to on-target reactions, such as digestion for protein identification. |
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