Determination of dimethylated arginines in human plasma by HPLC with fluorescent detection

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Authors

KLEPÁRNÍK Martin TOMANDL Josef

Year of publication 2007
Type Article in Proceedings
Conference Book of Abstracts
MU Faculty or unit

Faculty of Medicine

Citation
Web Advances in chromatography and electrophoresis 2007 & Chiranal 2007
Field Analytic chemistry
Keywords assymetric dimethylarginine; HPLC
Description Asymmetric dimethylarginine (ADMA) is a posttranslationally modified form of arginine that is generated in all types of cells during the process of degradation methylated proteins. We report here development of a new HPLC method for the determination of ADMA using fluorescent detection. After solid-phase extraction procedure and evaporation of the eluate under gentle stream of nitrogen the reconstituted samples were derivatised with an ortho-phthaldialdehyde reagent containing 3-mercaptopropionic acid. Formed derivatives were analysed on a Hydro-RP column using isocratic elution and fluorescence detection.
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