HPLC determination of midazolam and its three hydroxy metabolites in perfusion medium and plasma from rats

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Authors

JUŘICA Jan DOSTÁLEK Miroslav KONEČNÝ Jiří GLATZ Zdeněk HADAŠOVÁ Eva TOMANDL Josef

Year of publication 2007
Type Article in Periodical
Magazine / Source Journal of Chromatography B
MU Faculty or unit

Faculty of Science

Citation
Web http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6X0P-4N4J320-4&_user=835458&_coverDate=06%2F01%2F2007&_alid=584152625&_rdoc=1&_fmt=summary&_orig=search&_cdi=7220&_sort=d&_st=4&_docanchor=&_ct=44&_acct=C000045159&_version=1&_urlVersion=0&_userid
Field Biochemistry
Keywords cytochrome P450; midazolam; 1'-hydroxymidazolam; 4-hydroxymidazolam; 1'4-dihydroxymidazolam; rat; perfusion; plasma; HPLC
Description A new, simple, rapid, sensitive, and repeatable isocratic reverse-phase HPLC method was developed and validated for simultaneous determination of midazolam and its main three hydroxylated metabolites, i.e. 1' hydroxymidazolam, 4-hydroxymidazolam, and 1',4-dihydroxymidazolam in rat liver perfusate and also plasma. Diazepam was used as an internal standard to ensure precision and accuracy of this method. This method is applicable for modeling and description of possible pharmacological interactions on rat (CYP3A1/2) or human (CYP3A4/5) cytochrome P450 enzymes.
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