Characterization of staphylococcal type strains by whole-cell protein analysis and (GTG)5-PCR fingerprinting.
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| Year of publication | 2006 |
| Type | Conference abstract |
| MU Faculty or unit | |
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| Description | A group of 47 type strains representing all validly described staphylococcal species and subspecies was characterized by whole-cell protein analysis by using sodium dodecylsulfate-polyacrylamide gel electrophoresis and rep-PCR fingerprinting with the (GTG)5 primer. All strains were well typeable and revealed unique fingerprints by both methods. The individual staphylococcal type strains were differentiated on the level of 74 % using the protein analysis, with exception of novobiocin-resistant species, and on the level of 68 % by using the (GTG)5-PCR fingerprinting. Generally, all subspecies of the individual species showed close protein profiles that were grouped into individual clusters. Similarly, but with a few exceptions, the individual subspecies were separated into the distinct clusters using the (GTG)5-PCR fingerprinting. No clear correlation between the clustering obtained by both methods and the phylogenetic relationships of the analysed species was observed. However, most of the phylogenetically closely related coagulase-negative novobiocin-resistant species revealed very similar protein patterns. Our results showed the whole-cell protein profile analysis as well as rep-PCR fingerprinting with the (GTG)5 primer as suitable typing tools for characterization of staphylococci and their combination could be a helpful approach for identification of staphylococci. Financial support from project FRVS 2658 is gratefully acknowledged. |
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