A new, sensitive method for enzyme kinetic studies of scarce glucosides.

Warning

This publication doesn't include Faculty of Economics and Administration. It includes Faculty of Science. Official publication website can be found on muni.cz.

Authors	MAZURA Pavel FOHLEROVÁ Radka BRZOBOHATÝ Břetislav KIRAN Nagavalli Subbanna JANDA Lubomír
Year of publication	2006
Type	Article in Periodical
Magazine / Source	JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS
MU Faculty or unit	Faculty of Science
Citation
Field	Genetics and molecular biology
Keywords	Amplex UltraRed reagent; beta-glucosidase; glucose assay; glucose-oxidase / peroxidase coupled reaction; phytohormone conjugation; zeatin-O-glucoside
Description	We have developed a rapid, accurate and sensitive assay for glucose that enabled us to measure the kinetic parameters for a cytokinin-O-glucoside-specific beta-glucosidase towards its natural substrate. Both the beta-glucosidase reaction and the detection reaction occur simultaneously in solution. Measurement is continuous and the reaction is measured nine times during three seconds.
Related projects:	Molecular basis of cell and tissue regulations Regulation of morphogenesis of plant cells and organs