A new, sensitive method for enzyme kinetic studies of scarce glucosides.

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Authors

MAZURA Pavel FOHLEROVÁ Radka BRZOBOHATÝ Břetislav KIRAN Nagavalli Subbanna JANDA Lubomír

Year of publication 2006
Type Article in Periodical
Magazine / Source JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords Amplex UltraRed reagent; beta-glucosidase; glucose assay; glucose-oxidase / peroxidase coupled reaction; phytohormone conjugation; zeatin-O-glucoside
Description We have developed a rapid, accurate and sensitive assay for glucose that enabled us to measure the kinetic parameters for a cytokinin-O-glucoside-specific beta-glucosidase towards its natural substrate. Both the beta-glucosidase reaction and the detection reaction occur simultaneously in solution. Measurement is continuous and the reaction is measured nine times during three seconds.
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