Invasion of ED-1+ macrophages and changes of molecular expression in the contralateral homologous DRG following unilateral sciatic nerve injury
| Authors | |
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| Year of publication | 2006 |
| Type | Article in Proceedings |
| Conference | Morphology 2006 |
| MU Faculty or unit | |
| Citation | |
| Field | Neurology, neurosurgery, neurosciences |
| Keywords | dorsal root ganglia - cellular changes - molecular changes |
| Description | Due to the highly symmetrical organization of the peripheral nervous system, changes on the injured side were often compared to the uninjured contralateral side as control. However, within the last years, a growing number of reports of bilateral effects after unilateral peripheral nerve injury has been published. Unilateral ligature of the sciatic nerve was performed aseptically under deep anesthesia in 12 rats. Further 6 rats were sham-operated, and 4 rats without operation were used as naive control. All animals were left to survive for 1, 2, and 4 weeks. The L4-L5 dorsal root ganglia (DRG) were removed from ipsi- and contralateral side, and cryostat sections were incubated for immunofluorescence detection of ATF3, pCREB, TNFa, TNFR1, IL-1b, IL-6, SDF-1, and CXCR4. Macrophages invading DRG were detected by ED-1 immunostaining. In comparison to the DRG from naive rats, the qualitative and quantitative changes of immunofluorescence staining for the above-mentioned molecules excluding ATF3 were observed not only in the ipsi-, but also in homologous contralateral DRG during 1 to 4 weeks from operation. Invasion of ED-1+ macrophages was statistically increased in both ipsi- and contralateral DRG only after 4 weeks. The results indicate that contralateral DRG not affected directly by nerve injury display cellular and molecular changes that may contribute to a misbalance of signals from peripheral structures to CNS. |
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