Komparativní genomová sekvenace (CGS) kmenů rodu Treponema.
| Title in English | Comparative Genome Sequencing in the genus Treponema. |
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| Authors | |
| Year of publication | 2006 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Treponema pallidum ssp. pallidum is an exclusive human pathogen and the causative agent of syphilis. Genus Treponema encompasses several closely related species/subspecies in which the levels of pathogenicity and invasivity to humans vary to a wide extent. Moreover, these organisms can not be differentiated morphologically or serologically. To obtain complete genome sequence of examined strains CGS found changes were combined with WGF results. We performed comparison of SS14, Samoan D and Cuniculi A strains to Nichols strain. Mapping phase of T. pallidum ssp. pallidum SS14 genome showed 20 hypervariable regions not suitable for further hybridization analysis. Resequencing array revealed 231 SNPs. We found altogether 320 SNPs, 11 deletions and 15 insertions (1-1255 bp in lenght). In T. pallidum ssp. pertenue Samoan D genome, 904 SNPs were identified. In T. paraluiscuniculi Cuniculi A genome 5933 SNPs were discovered. |
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