Determination of midazolam and its metabolites in rat plasma and perfuse medium by HPLC

• Authors: JUŘICA Jan; TOMANDL Josef; DOSTÁLEK Miroslav; HADAŠOVÁ Eva
• Year of publication: 2006
• Type: Article in Proceedings
• Conference: CD of the abstracts of the 29th international symposium on capillary chromatography
• MU Faculty or unit: Faculty of Medicine
• Field: Biochemistry
• Keywords: Cytochrome P450; Midazolam; 1 Hydroxymidazolam; 4 Hydroxymidazolam; 1 4 Dihydroxymidazolam; Rat; Perfuzate; Plasma
• Description: A new isocratic reverse-phase high-performance liquid chromatography utilising ultraviolet detection for the simultaneous determination of midazolam and its three main hydroxymetabolites in rat plasma and rat liver perfuse medium is described. The present method offers various advantages over the currently used HPLC methods, including speed, sensitivity and reproducibility. Moreover, it may be applicable for modelling and description of possible pharmacological interactions on rat or human isoforms of cytochrome P450.

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