PIN proteins perform a rate-limiting function in cellular auxin efflux

Petrášek, J.; Mravec, J.; Bouchard, R.; Blakeslee, J.; Abas, M.; Seifertová, D.; Wisniewska, J.; Tadele, Z.; Čovanová, M.; Dhonukshe, P.; Skůpa, P.; Benková, E.; Perry, L.; Křeček, P.; Lee, O.R.; Fink, G.; Geisler, M.; Murphy, A.; Luschnig, C.; Zažímalová, E.; Friml,  Jiří

PIN proteins perform a rate-limiting function in cellular auxin efflux

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Authors	PETRÁŠEK J. MRAVEC J. BOUCHARD R. BLAKESLEE J. ABAS M. SEIFERTOVÁ D. WISNIEWSKA J. TADELE Z. ČOVANOVÁ M. DHONUKSHE P. SKŮPA P. BENKOVÁ E. PERRY L. KŘEČEK P. LEE O.R. FINK G. GEISLER M. MURPHY A. LUSCHNIG C. ZAŽÍMALOVÁ E. FRIML J.
Year of publication	2006
Type	Article in Periodical
Magazine / Source	Science
MU Faculty or unit	Faculty of Science
Citation
Field	Genetics and molecular biology
Keywords	auxin transport; auxin efflux; PIN proteins; Arabidopsis
Description	Intercellular flow of the phytohormone auxin underpins multiple developmental processes in plants. Plant-specific PIN proteins and several PGP transporters are crucial factors in auxin transport-related development, yet PINs molecular function remains unknown. Here we show that PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. Conditional gain-of-function alleles and quantitative measurements of auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the action of PINs in auxin efflux is distinct from PGPs, rate-limiting, specific to auxins and sensitive to auxin transport inhibitors. This suggests a direct involvement of PINs in catalyzing cellular auxin efflux.
Related projects:	Molecular basis of cell and tissue regulations Regulation of morphogenesis of plant cells and organs