Embryonic stem cell-derived microvesicles reprogram hematopoietic progenitors: evidence for horizontal transfer of mRNA and protein delivery

Dvořák,  Petr; Ratajczak, J.; Miekus, K.; Kucia, M.; Zhang, J.; Reca, R.; Ratajczak, MZ.

Embryonic stem cell-derived microvesicles reprogram hematopoietic progenitors: evidence for horizontal transfer of mRNA and protein delivery

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Authors	DVOŘÁK Petr RATAJCZAK J. MIEKUS K. KUCIA M. ZHANG J. RECA R. RATAJCZAK MZ.
Year of publication	2006
Type	Article in Periodical
Magazine / Source	Leukemia
MU Faculty or unit	Faculty of Medicine
Citation
Field	Physiology
Keywords	microvesicles; embryonic stem cells; stem cell expansion; mRNA transfer; Oct-4
Description	Membrane-derived microvesicles (MV) are released from the surface of activated eukaryotic cells and exert pleiotropic effects on surrounding cells. We found that MV derived from embryonic stem cells enhanced survival and improved expansion of murine hematopoietic stem cells, upregulated the expression of early pluripotent (Oct-4, Nanog, Rex-1) and early hematopoietic stem cell (Scl, HoxB4, GATA 2) markers in these cells, and induced phosphorylation of MAPK and Akt. We postulate that embryonic stem cell-derived MVs may efficiently expand hematopoietic stem cells by stimulating them with MV expressed ligands as well as increase their pluripotency after horizontal transfer of mRNA.
Related projects:	Centrum chemické genetiky