Embryonic stem cell-derived microvesicles reprogram hematopoietic progenitors: evidence for horizontal transfer of mRNA and protein delivery

Warning

This publication doesn't include Faculty of Economics and Administration. It includes Faculty of Medicine. Official publication website can be found on muni.cz.
Authors

DVOŘÁK Petr RATAJCZAK J. MIEKUS K. KUCIA M. ZHANG J. RECA R. RATAJCZAK MZ.

Year of publication 2006
Type Article in Periodical
Magazine / Source Leukemia
MU Faculty or unit

Faculty of Medicine

Citation
Field Physiology
Keywords microvesicles; embryonic stem cells; stem cell expansion; mRNA transfer; Oct-4
Description Membrane-derived microvesicles (MV) are released from the surface of activated eukaryotic cells and exert pleiotropic effects on surrounding cells. We found that MV derived from embryonic stem cells enhanced survival and improved expansion of murine hematopoietic stem cells, upregulated the expression of early pluripotent (Oct-4, Nanog, Rex-1) and early hematopoietic stem cell (Scl, HoxB4, GATA 2) markers in these cells, and induced phosphorylation of MAPK and Akt. We postulate that embryonic stem cell-derived MVs may efficiently expand hematopoietic stem cells by stimulating them with MV expressed ligands as well as increase their pluripotency after horizontal transfer of mRNA.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.