Molekulární interakce kolicinu U se svým imunitním proteinem.

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Title in English Molecular interaction of colicin U with its immunity protein
Authors

JANALÍKOVÁ Magda ŠMAJS David

Year of publication 2005
Type Article in Proceedings
Conference Chemické listy 99
MU Faculty or unit

Faculty of Medicine

Citation
Field Genetics and molecular biology
Keywords colicin; immunity protein; molecular recognition
Description The colicin-immunity protein recognition is a suitable model for studying protein-protein interactions. There are 34 amino acid residues different between sequences of colicin U a Y in these pore-forming bactericidal domains. Almost all different amino acid residues in the pore forming domain were mutated by site-directed mutagenesis to the corresponding sequence of colicin Y (U). More than 40 mutant colicins were prepared and their toxic effect was tested on the sensitive bacteria transformed with plasmid harboring either cui (colicin U immunity) gene or cyi (colicin Y immunity) gene. Colicin U variants with point mutations in the hydrophobic hairpin, F576Y and G580V, were significantly less recognized by Cui, in addition to colicin U with K609R mutation localized downstream of hydrophobic hairpin. In the colicin Y pore-forming domain, Y586 and A589 were identified as interaction sites with Cyi in the hydrophobic hairpin, in addition to A577-I578 in the helix 8 of colicin Y pore forming domain.
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