Molekulární interakce kolicinu U se svým imunitním proteinem.
| Title in English | Molecular interaction of colicin U with its immunity protein |
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| Authors | |
| Year of publication | 2005 |
| Type | Article in Proceedings |
| Conference | Chemické listy 99 |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | colicin; immunity protein; molecular recognition |
| Description | The colicin-immunity protein recognition is a suitable model for studying protein-protein interactions. There are 34 amino acid residues different between sequences of colicin U a Y in these pore-forming bactericidal domains. Almost all different amino acid residues in the pore forming domain were mutated by site-directed mutagenesis to the corresponding sequence of colicin Y (U). More than 40 mutant colicins were prepared and their toxic effect was tested on the sensitive bacteria transformed with plasmid harboring either cui (colicin U immunity) gene or cyi (colicin Y immunity) gene. Colicin U variants with point mutations in the hydrophobic hairpin, F576Y and G580V, were significantly less recognized by Cui, in addition to colicin U with K609R mutation localized downstream of hydrophobic hairpin. In the colicin Y pore-forming domain, Y586 and A589 were identified as interaction sites with Cyi in the hydrophobic hairpin, in addition to A577-I578 in the helix 8 of colicin Y pore forming domain. |
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