Reversed Phase HPLC Separation of Polycyclic Aromatic Nitrogen Heterocycles controlled by mobile phase pH

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Authors

ŠVÁBENSKÝ Radim ŠIMEK Zdeněk KOČÍ Kamila

Year of publication 2005
Type Article in Proceedings
Conference Abstract Book- 29th International Symposium on High Performance Liquid Phase Separations and Related Techniques
MU Faculty or unit

Faculty of Science

Citation
Field Analytic chemistry
Keywords HPLC ; Polycyclic Aromatic Nitrogen Heterocycles; PANH
Description Retention behaviour of individual polycyclic aromatic nitrogen heterocycles (PANHs) was studied at different chromatographic conditions. Mixture of phosphate buffer/organic modifier was used as mobile phase in isocratic and gradient mode. Effect of different pH mobile phase on retention of individual PANHs has been investigated to describe retention changes of PANHs as a function of acid-base properties. Different buffer concentration as a component of mobile phase was used for ionic strength effect study. PANHs are widely spread in the environment. They are formed and released into the environment from similar sources like other toxic polycyclic aromatic compounds [1]. The possibility of determination of PANHs in a mixture with polycyclic aromatic hydrocarbons (PAHs) on octadecyl stationary phase without pre-separation steps is demonstrated. The large variability in mobile phase composition allows controlling properties of HPLC separation and determination of PANHs in particular environmental samples.
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