Optimalizácia prípravy a spracovanie molekulárne cytogenetických preparátov pre spektrálne karyotypovanie

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Title in English The preparation and treatment optimization of molecular cytogenetic slides for spectral karyotyping
Authors

LOJA Tomáš KUGLÍK Petr VESELSKÁ Renata

Year of publication 2005
Type Conference abstract
MU Faculty or unit

Faculty of Science

Citation
Description The aim of the work was to optimize the most important steps in a preparation of molecular cytogenetic slides for SKY analysis. The optimization included: looking for optimal protease concentration, pretreatment and its comparison, aging types influence on chromosome structure and shape. The result was a suggestion of optimal protocol for SKY. Practical possibilities of SKY were shown on material from cytogenetic investigation.
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