Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Identification of Fluorescently Labeled Peptides for Optimization of Laser Induced Fluorescence Detection

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Authors

VRÁBEL Patrik TÁBORSKÝ Petr RYVOLOVÁ Markéta HAVEL Josef PREISLER Jan

Year of publication 2004
Type Article in Proceedings
Conference Journal of Peptide Science, 3rd International and 28th European PEPTIDE Symposium
MU Faculty or unit

Faculty of Science

Citation
Web http://www3.interscience.wiley.com/cgi-bin/jissue/109608640
Field Analytic chemistry
Keywords electrophoresis; MALDITOF MS; fluorescence; derivatization; rhodamine B isothiocyanate; peptides
Description Laser induced fluorescence detection (LIF) is an effective for the sensitive determination of biologically important molecules, such as peptides and proteins, after Capillary Electrophoresis (CE) separation. In this work we demonstrate preliminary results obtained using CELIF and matrix assisted laser desorption/ionization mass spectrometry (MALDI MS) to investigate derivatization of model peptides with rhodamine-based reagents.
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