Gene expression in colorectal carcinoma determined by cDNA microarrays using a combination of several evaluation approaches.

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Authors

JANSOVÁ Eva KRONTORÁD Petr SVOBODA Zbyněk KOUTNÁ Irena PAVLÍK Tomáš KOZUBEK Michal JAROŠOVÁ Marie ŽALOUDÍK Jan KOZUBEK Stanislav

Year of publication 2004
Type Article in Proceedings
Conference Biophysics of the Genome
MU Faculty or unit

Faculty of Informatics

Citation
Field Genetics and molecular biology
Keywords microarrays
Description We have compared colorectal carcinoma tissues with parallel samples of epithelial tissue and identified, by means of Human 1.7K cDNA microarrays, a set of genes with significantly altered expression in 12 patients. We used a combination of several approaches of microarray data analysis to be sure that the results were reliable. Although we have found differences between the results obtained by various image analysis software packages and using different statistical tools, we have identified the group of 22 genes with significantly altered expression in colorectal carcinoma tissue as compared with epithelial tissue using all evaluation approaches. Hierarchical clustering showed the possibility of dividing the patients into two groups according to the presence of metastases in regional lymph nodes. We have proposed 6 genes as potential markers for the detection of the presence of regional metastases in patients. We suppose that the proposed advance in the analysis of microarray data could lead to easier, quicker and more effective diagnostics of various diseases dependent on changes in the expression profile.
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