Determination of Midazolam from Perfuse Medium as a Probe Substrate of Cytochrome P450 3A1/2 Activity in Rats
| Title in English | DETERMINATION OF MIDAZOLAM FROM PERFUSE MEDIUM AS A PROBE SUBSTRATE OF CYTOCHROME P450 3A1/2 ACTIVITY IN RATS |
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| Authors | |
| Year of publication | 2004 |
| Type | Article in Proceedings |
| Conference | ACTA UNIVERISTATIS PALACKIANAE OLOMUCENSIS FACULTAS RERUM NATURALIUM |
| MU Faculty or unit | |
| Citation | |
| Field | Pharmacology and pharmaceutical chemistry |
| Keywords | Midazolam; CYP-450 |
| Description | The aim of the project was to develop HPLC method for determination of midazolam and its three metabolites, 1- and 4-hydroxymidazolam and 1,4-dihydroxymidazolam from perfuse rat liver medium. Hydroxylation of midazolam is used as a valuable tool for measurement of metabolic activity of the 3A1/2 isoform of cytochrome P-450 in rats (CYP3A1/2)This method is simple, rapid, sensitive and applicable for modeling and description of possible pharmacological interactions on rat CYP3A1/2 or it`s human orthologue CYP3A4/5, respectively. |
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