Isolation of genomic DNA using magnetic cobalt ferrite and silica particles

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Authors

PRODĚLALOVÁ Jana RITTICH Bohuslav ŠPANOVÁ Alena PETROVÁ Kateřina BENEŠ Milan

Year of publication 2004
Type Article in Periodical
Magazine / Source Journal of Chromatography A
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords Silica particles; Cobalt ferrite particles; DNA; Polymerase chain reaction
Description Two types of particles were used: silica and cobalt ferrite (unmodified or modified with a reagent containing weakly basic aminoethyl groups, aminophenyl groups, or alginic acid). DNA from chicken erythrocytes and DNA isolated from bacteria Lactococcus lactis were used for testing of adsorption/desorption properties of particles. The cobalt ferrite particles modified with different reagents were used for isolation of PCR-ready bacterial DNA from different dairy products.
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