Ribotyping and biotyping of <I>Staphylococcus sciuri</I> species group from humans
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| Year of publication | 2004 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Members of the Staphylococcus sciuri species group are infrequently isolated from human clinical material. In our study a collection of 72 clinical isolates originating from Czech Republic and 6 reference type strains of S. sciuri complex were characterized by ribotyping and biotyping. All strains were ribotyped using EcoRI restriction enzyme and probe complementary to 16S and 23S rRNA. The relatedness of ribotypes was evaluated by using the Dice coefficient and clustered by UPGMA method. Biotyping was performed with the API Staph and ID 32 Staph kits supplemented by conventional tests key for this bacterial group and by whole-cell protein profiles analysis (1D SDS-PAGE). Biochemical identification of isolates from S. sciuri species group based on commercial kits was often uncertain due to variations observed in some tests and required additional testing. All isolates and type strains were clearly distinguished by ribotyping according to their specific ribotypes at the species or subspecies level. The phenotypically close species S. sciuri and S. lentus were separated by ribotyping and SDS-PAGE analysis giving accurate identification of S. lentus. The isolates belonging to the S. sciuri species formed six homogeneous clusters using their EcoRI hybridization patterns in accordance with subspecies taxonomy while SDS-PAGE results indicated patterns generally applicable for strain typing only. Analysis of human isolates showed that identification kits or SDS-PAGE were less powerful for species and subspecies identification of S. sciuri species group. On the base of ribotype pattern types we proved in human clinical material S. lentus, S. sciuri subsp. sciuri, S. sciuri subsp. carnaticus and S. sciuri subsp. rodentium. Ribotyping appeared to be a good tool for differentiation among taxa of S. sciuri complex. |
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