Capillary Electrophoresis of Bacteriophage 812 Proteins Combined with MALDI Mass Spectrometry

• Authors: PREISLER Jan; VRÁBEL Patrik; NOVÁKOVÁ Jaromíra; SPĚŠNÝ Michal; ZDRÁHAL Zbyněk; KONEČNÁ Hana; KRÁSENSKÝ Pavel; EYER Luděk; DOŠKAŘ Jiří; PANTŮČEK Roman; FORET František; KLEPÁRNÍK Karel
• Year of publication: 2004
• Type: Conference abstract
• MU Faculty or unit: Faculty of Science
• Description: The alarming increase in antibiotic-resistant bacteria has renewed interest in bacteriophage therapy of various human infections, such as postoperative staphylococcal wound infections or anthrax. In this work, SDS gel electrophoresis was used for the protein separation followed by tryptic digestion and ESI or MALDI analysis.Additionally, we have proposed a new approach to the analysis of the phage proteins, especially low abundant proteins, such as lytic enzymes. Protein and peptide separations were performed with capillary electrophoresis (CE) and a new universal interface has been developed to couple CE with MALDI MS. A method of protein microdigestion on MALDI target is being studied and will be used for protein identification.

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