4-nitrocatechol and 5-nitroguaiacol degradation by Rhodococcus opacus C6-1 isolated from soil

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Authors

KOTOUČKOVÁ Ludmila NEČA Jiří MORÁVKOVÁ Monika ČECHOVÁ Leona NĚMEC Miroslav

Year of publication 2003
Type Article in Proceedings
Conference The 1st FEMS Congress of European Microbiologists, Abstract Book.
MU Faculty or unit

Faculty of Science

Citation
Field Microbiology, virology
Keywords Rhodococcus; 5-nitroguaiacol; 4-nitrocatechol; degradation; automatic spectrophotometry
Description Degradation of 4-nitrocatechol and 5-nitroguajacol by a Rhodococcus species isolated from soil was reported. Degradation of 4-nitrocatechol is not connected with capability of degrade 4-nitrophenol in the case of the strain C6-1, in contrast to known bacteria degrading 4-nitrocatechol. 4-Nitrocatechol is used as sole source of carbon and nitrogen in concentration up to 0.5 mol.l-1. The yellow colour of substrate allows the using of method of automatic spectrophotometry (BIOSCREEN C) for detailed determination of degradation profile of 4-nitrocatechol. Resting cells experiments were used for determination of basic kinetic parameters of reactions.
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