Electrochemical determination of lead and glutathione in a plant cell culture

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Authors	VACEK Jan PETŘEK Jiří KIZEK René HAVEL Ladislav KLEJDUS Bořivoj TRNKOVÁ Libuše JELEN František
Year of publication	2004
Type	Article in Periodical
Magazine / Source	Bioelectrochemistry
MU Faculty or unit	Faculty of Science
Citation
Field	Electrochemistry
Keywords	Lead; Pb(II)-EDTA; glutathione; metallothionein; Voltammetry; Mercury electrode; Brdicka reaction
Description	In this paper we established DPASV as the tool for the analysis of lead in plant cell culture. For the calibration procedure, lead in (Pb-EDTA) chelate has been used. The detection limit of lead was at 500 pM in phosphate buffer (pH 5.5), and 100 nM in prepared cells intracellular extract. For the determination of cystein-rich peptides, voltammetry in differential mode in cobalt(III)-containing ammonia buffer (Brdicka reaction) was used.
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