Passive penetration of nitrate through the plasma membrane of Paracoccus denitrificans and its potentiation by the lipophilic tetraphenylphosphonium cation

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Authors

KUČERA Igor

Year of publication 2003
Type Article in Periodical
Magazine / Source Biochimica et Biophysica Acta
MU Faculty or unit

Faculty of Science

Citation
Web http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T1S-47XFNFG-1&_user=835458&_handle=B-WA-A-A-AD-MsSAYZW-UUW-AUYBDCVAAE-AUYABBVEAE-BVWUCBDYU-AD-U&_fmt=full&_coverDate=03%2F06%2F2003&_rdoc=13&_orig=browse&_srch=%23toc%234898%232003%23984429999%23
Field Biochemistry
Keywords nitrate transport; nitrate reductase; permeant ion; ion pair; uncoupler; bacterium
Description The inhibition by phenylglyoxal of the methyl-viologen-linked nitrate reductase disappears if tetraphenylphosphonium cation is added to the assay medium. The paper demonstrates by several independent approaches that this effect results from an increased anion permeability of the membrane.
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