Analýza proteomu polyvalentního bakteriofága 812
| Title in English | Proteome analysis of the polyvalent bacteriophage 812 |
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| Authors | |
| Year of publication | 2004 |
| Type | Article in Proceedings |
| Conference | VIII. Pracovní setkání biochemiků a molekulárních biologů. Sborník abstraktů |
| MU Faculty or unit | |
| Citation | |
| web | http://orion.chemi.muni.cz/Setkani/setkani8/index.htm |
| Field | Genetics and molecular biology |
| Keywords | bacteriophage proteomics; MALDI-TOF; therapeutic bacteriphage; Staphylococcus bacteriophage |
| Description | Virulent and polyvalent staphylophage 812, a member of Myoviridae family, exhibits strong lytic activity on a broad host range of Staphylococcus species, thus being a suitable candidate for treatment of staphylococcal infections. Genome of this phage is represented by linear 145 kb dsDNA. Our study was focused on genome and proteome comparison of 812 wild-type phage, its host-range mutants and related phages U16, 131 and SK311 with the aim to explain molecular basis of differences in their host-range. Comparison of the phage genomes revealed several rearrangements of DNA sequences as well as single nucleotide substitutions in different parts of the genomes. Nucleotide insertions and deletions of various size (~1 to 3 kb) were most frequently found in non-coding terminal genomic regions in phages with different host range. We tried to find possible correlations between mutational changes on DNA and proteome composition of the phages. After SDS-PAGE separation of phage virions digests 15 protein bands with the mass ranging from 15 to 80 kDa were identified. Twelve bands were excised for further analysis by in-gel digestion with consecutive MALDI-TOF MS. |
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