Electrochemical Detection of Interactions of Echinomycin with DNA at Mercury Film Electrodes (MFE).
| Authors | |
|---|---|
| Year of publication | 2001 |
| Type | Article in Proceedings |
| Conference | Book of Abstracts, US-CZ Workshops on Electrochemical Sensors Prague 2001 |
| MU Faculty or unit | |
| Citation | |
| Field | Biophysics |
| Keywords | DNA-echinomycin interaction; electrochemical impedance spectroscopy;EIS; DNA adsorption; mercuiry film electrodes; |
| Description | The dependence of the impedance Z and/or differential capacitance C of the electrode double layer on electrode potential E and on frequency in solutions of echinomycin with single stranded (ss) and double-helical (double stranded, ds) DNA was measured. Echinomycin gives a pseudocapacitance redox peak on the C-E curves at the potential of -0.53 V. This peak is observed with the dsDNA-echinomycin comlex as well, but not with ssDNA treated by echinomycin. The capacitance measurements can thus distinguish with the aid of bis-intercalator echinomycin the ss and ds form of DNA adsorbed at the electrode surface. This promise to use impedance measurements for detection of dsDNA in hybridization reactins. |
| Related projects: |