Využití preparativní isoelektrické fokusace s imobilizovaným gradientem při purifikaci proteinů

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Title in English	The use of preparative isoelectric focusation with immobilized gradient for protein purification
Authors	DRÁBKOVÁ Lenka JANICZEK Oldřich MANDL Martin
Year of publication	2002
Type	Article in Periodical
Magazine / Source	Chemické listy
MU Faculty or unit	Faculty of Science
Citation
Field	Biochemistry
Keywords	protein purification; isoelectric focusation; Acidithiobacillus
Description	A multichamber electrofocusing unit PI 8 IsoPrime using membranes with immobilized pH was applied. Proteins of an egg albumen were used as a model system. pH values were 4.5, 5.5, 8,0 and 9.5. Separation ability was tested using electroforesis. The apparatus was also used for soluble and membrane immobilized cytochrome separation from Acidithiobacillus ferrooxidans.
Related projects:	Acidification processes in sulfide wastes Biomolecular Structure-function Relationships and their role in the Metabolism